Flow Resources

Flow Cytometry Basics

Introduction to Flow Cytometry – Basics Guide from Bio-Rad
Fluorescence Tutorials from ThermoFisher

Panel Design

Optimized Multicolor Immunofluoresence Panels from Cytometry Part A
FluoroFinder search antibodies from all vendors in one place
Antigen Density for Human and Murine Surface Markers from Bio-Rad

Spectra Viewers

FPbase Spectra Viewer easily view spectra of multiple dyes or fluorescent proteins
Cytek Full Spectrum Viewer for spectral panel design
Spectrum Viewer from BD Biosciences
Spectra Viewer from ThermoFisher
Fluorescence Spectra Analyzer from BioLegend

Data analysis Software

FlowJo Recorded Webinars

Dimensionality Reduction and Population Discovery

FlowJo exchange free downloads of FlowJo plugins
Comparison of clustering methods for high-dimensional single-cell flow and mass cytometry data
FlowSOM FlowJo Plugin Tutorial
FlowSOM: Using self-organizing maps for visualization and interpretation of cytometry data

Specialized Applications

Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death
RNA Flow Cytometry Using the Branched DNA Technique