Tissue Culture Core Facility (TCCF)

Oligo Orders

Oligo Orders

  • Vendor Representative: Karen Juntunen van Delft - 281-210-2255 | karen.juntunen@sial.com

    Investigators and researchers can order oligo sequences from Sigma-Genosys directly through TCCF. This process does not require a PO# and the oligos may be picked up in the same manner as other products purchased in the facility. SHIPPING IS FREE.

    To register, click on the link below and enter the registration code: _UTMBOLIGOS
    Sigma-Genosys link: http://www.sigmaaldrich.com/pipeline   

    Registration Instructions Download 
    This will prompt you for some necessary information. Once you have completed this, allow 24 hours to process the registration and you will receive an email confirming that registration is complete.

    Researchers will receive an email confirmation when the order has been received by Sigma-Genosys. Once an order is complete it is shipped to the TCCF. Researchers will be notified via email when an order is ready for pickup at the facility. A shipment table of expectant turn-around times is indicated below. (Estimated turn-around times do not include weekends or holidays.)

    Download Information on Same Day Custom Oligo Orders 

    Scale (μmole) Desalt Cartridge HPLC PAGE
    0.03 (3 O.D. Select) 1-2 days N/A N/A N/A
    0.05 1-2 days 1-2 days 5 days or less 3 days or less
    0.2 1-2 days 1-2 days 5 days or less 3 days or less
    1.0 1-2 days 1-2 days 5 days or less 3 days or less
    Oligos, <40bp Qualifies for Same Day Service. Order by 12 PM and receive product the next day (see above flyer for specs)
    Long Oligos, 121bp to 180bp Please contact Karen Juntunen van Delft @ 281-210-2255 or karen.juntunen@sial.com
    Greater than 1.0μmole 10 days or less
    Large quantity Inquire
    Post-synthesis modified 7-10 days
    All other modifications 5-7 days or less

    The table below indicates the products and pricing available through the TCCF/Sigma-Genosys partnership.

    Description 3 OD* Select (0.025 μmole) 0.05 μmole 0.2 μmole 1.0 μmole
    Base Charge $0.11 $0.15 $0.35 $0.80
    RP1 N/A $5.00 $5.00 $8.00
    PAGE N/A $25.00 $35.00 $59.00
    HPLC N/A $25.00 $35.00 $59.00
    5' Biotin N/A $20.00 $20.00 $45.50
    5' Phosphorylation N/A $10.00 $10.00 $20.00
    5' Fluorescein N/A $20.00 $20.00 $45.00
    5' 6-FAM N/A $30.00 $30.00 $45.00
    5'HEX or TET N/A $35.00 $35.00 $48.00
    5' Amine C6 N/A $10.00 $10.00 $20.00

    For further pricing on items not listed please contact the Tissue Culture Core Facilty.

    Each oligo sequence is physically tested using one or more of the following techniques: MALDI-TOF mass spectrometry, Electrospray lonization mass spectrometry, and Capillary Electrophoresis. Final yields are determined using UV absorbance at OD260. A Quality Assurance Certificate will be provided for every order indicating the yield, melting temperature, MW and µg/OD of each oligo sequence. Sigma-Genosys is ready to provide mass spec traces for all sequences that are desalted, non-modified, and <50 nucleotide bases upon request. The Tissue Culture Core Facility adds a 15% surcharge to all completed orders. Phone: (409) 772-4903

  • Campus Vendor Representative: Ric Sugarek - rsugarek@idtdna.com

    Investigators and researchers can order oligo sequences from IDT directly through the Tissue Culture Core Facility. This process does not require a PO# and the TCCF handles all financial aspects through in-house billing. The TCCF provides technical support for portal users throughout the ordering process and the oligos may be picked up in the same manner as other products purchased in the facility. Researchers will receive an email confirmation when the order has been received by IDT. Once an order is complete it is shipped to the TCCF. Researchers will be notified via email when an order is ready for pickup at the facility. The TCCF applies a 10% surcharge on all orders made through the IDT portal for services rendered.

    The table below indicates the pricing available through the TCCF/IDT partnership.

    Description

    3 OD (25 nmole)

    100 nmole

    250 nmole

    Base Charge

    $0.12

    $0.35

    $0.55

    Description

    1 μmole

    5 μmole

    10 μmole

    Base Charge

    $1.00

    $7.22

    $13.60

    Setting up your TCCF/IDT portal account is a quick and easy process.

    1. Select the link below
    2. First time users click "Set Up a New Account": http://www.idtdna.com/utmb-tccf
      1. Create a login name and password
      2. Enter personal information
    3. Returning users log in using username and password
    4. Researchers can order via email at: https://www.idtdna.com/pages/home/how-to-order
    5. Download easy to follow instructions for sign up and ordering here